Will this work in a PCI-E 3.0 motherboard?

Yes, the AORUS Gen 4 SSD is backward compatible with PCIe 3.0 motherboards, although you will not be able to utilize its full speed capabilities as PCIe 3.0 has lower bandwidth than PCIe 4.0.

Questions & Answers: GIGABYTE AORUS Gen4 M.2 2280 2TB PCI-Express 4.0 x4, NVMe 1.3 3D TLC Internal Solid State Drive (SSD) GP-AG42TB

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GIGABYTE AORUS Gen4 M.2 2280 2TB PCI-Express 4.0 x4, NVMe 1.3 3D TLC Internal Solid State Drive (SSD) GP-AG42TB

Form Factor: M.2 2280
Interface: PCI-Express 4.0x4, NVMe 1.3
Sequential Read Speed : up to 5000 MB/s
Sequential Write speed : up to 4400 MB/s
Wear Leveling, Over-Provision technologies
TRIM & S.M.A.R.T supported

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